Sunday 23 February 2014

Drug Induced Liver Disease (Hepatotoxicity)-Chapter 11-HISTOLOGICAL PATTERN IN DRUG-INDUCED LIVER DISEASE Part-5

Figure.11.13:-Phospholipidosis. Formation of lysosomal inclusion bodies due to accumulation of amiodarone. Electron micrograph.
Figure.11.14:- Sinusoidal obstruction syndrome. Endothelial injury in small hepatic venules leads to luminal occlusion due to endothelial swelling and thrombosis, and results in sinusoidal dilatation and congestion.
Figure.11.5:- Peliosis. The hepatic parenchyma contains blood-filled cavities that lack an endothelial lining (arrows).

Figure.11.16:- Stellate cell (Ito cell) lipidosis. Fat-laden stellate cells showing multiple lipid vacuoles with indentation of the nucleus. Note the location of Ito cells along the sinusoids in the space of Disse.
Other patterns
Stellate cell lipidosis
Hepatic stellate cells (Ito cells) are modified fibroblasts that store lipids and vitamin A in the normal liver. They are located in the space of Disse between the sinusoidal endothelium and the hepatocytes but generally are not easily visible. In certain conditions, especially hypervitaminosis A, excessive lipid gets stored in the stellate cells (stellate cell lipidosis, fig 16). The nuclei of stellate cells are crescent shaped, dark staining, and indented by the lipid droplets. Thin strands of cytoplasm separate the lipid droplets. These lipid-laden cells easily can be mistaken for hepatocytes with steatosis. Their characteristic morphology and location along the sinusoids between the hepatic plates distinguishes them from steatotic hepatocytes.
Hypervitaminosis A results from excess dietary/supplementary vitamin A intake or use of oral/topical retinoids (such as etretinate for acne). Stellate cell lipidosis also has been reported with methotrexate, valproate and steroids, as well as in other clinical settings such as cholestasis, alcoholic liver disease and hepatitis C.
Cytoplasmic inclusions
Ground glass change in the cytoplasm occurs in a minority of patients with hepatitis B and is characterised by pale eosinophilic cytoplasmic inclusions in hepatocytes. Similar changes (often termed “pseudo ground glass change”, fig 17) can be seen with drugs such as cyanamide, a drug used in alcohol treatment programs. This phenomenon has also been described with other drugs such as barbiturates and diazepam, diabetic patients on insulin, and transplant patients on multiple immunosuppressive drugs such as steroids, tacrolimus and mycophenolate mofetil. Similar to hepatitis B.


Figure.11.17:- Ground-glass hepatocytes. This change can be seen with drugs such as cyanamide (used for treating alcohol abuse), diazepam and barbiturates, and in patients on insulin or intravenous glucose therapy                                                                     
 

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